reverse transcription reagent prime scripttm rt reagent kit (TaKaRa)
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reverse transcription reagent prime scripttm rt reagent kit
Reverse Transcription Reagent Prime Scripttm Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scripttm+reverse+transcription+kit/PrimeScript+RT+Reagent+Kit/pm41837479-39-83-94
Average 99 stars, based on 88195 article reviews
Reverse Transcription Reagent Prime Scripttm Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scripttm+reverse+transcription+kit/PrimeScript+RT+Reagent+Kit/pm41837479-39-83-94
Average 99 stars, based on 88195 article reviews
reverse transcription reagent prime scripttm rt reagent kit - by Bioz Stars,
2026-09
99/100 stars
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CCK-8 Assay:Article Title: Cyasterone Improves Mitochondrial Function and Protects against Knee Osteoarthritis by Activating PPARγ. Article Snippet: Beijing University of Chinese Medicine Shenzhen Hospital (Longgang), Shenzhen, 518172, P.R.. China; Key Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510080, P.R.. China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science and Innovation, CAS Ltd, Fifth Affiliated Hospital, Guangzhou Medical University, P.R. Article Title: Huo Xue Tong Luo capsule epigenetically activates β-catenin, promotes Prx1-lineage cells proliferation and endochondral bone formation during fracture healing Article Snippet: The animals were housed in the SPF-grade experimental animal center of the First Affiliated Hospital of Guangzhou University of Chinese Medicine, with the license number SCXK (Guangdong) 2023-0092. .. Small animal X-Ray imager (Faxitron X-Ray, Tucson, AZ, USA); Small animal micro-CT (viva CT 40); Biosafety cabinet, cell culture incubator, ultra-low- temperature freezer, Varioskan LUX multi-function microplate reader (Thermo); Quantitative fluorescence PCR instrument (BioRAD); Polyvinylidene fluoride (PVDF) membrane (Millipore); Paraffin microtome, cryostat microtome, image information acquisition system (Leica); Fetal bovine serum (Gibco Cat NO. 10091-48); penicillin/streptomycin (Cytiva, HyClone Cat NO. SV30010), 0.25% trypsin (Cytiva, HyClone Cat NO. SV30031.01); Huo Xue Tong Luo capsule (Approval No. Z20071224 of Guangdong Provincial Medicine Preparation, the First Affiliated Hospital of GZUCM); β-Glycerophosphate disodium salt (Solarbio, Cat NO. G8100); Dexamethasone (Sigma, Cat NO. D4902-100MG); Ascorbic acid (Sangon Biotec, Cat NO. A610021); Alizarin red (Solarbio, Cat NO. G1450); ALP (Sigma, Cat NO. 86 C-1KT); Cell Counting Kit-8 (CCK-8) for cell proliferation detection (Solarbio, Cat NO. CA1210); Fluorescence:Article Title: Cyasterone Improves Mitochondrial Function and Protects against Knee Osteoarthritis by Activating PPARγ. Article Snippet: Beijing University of Chinese Medicine Shenzhen Hospital (Longgang), Shenzhen, 518172, P.R.. China; Key Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510080, P.R.. China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science and Innovation, CAS Ltd, Fifth Affiliated Hospital, Guangzhou Medical University, P.R. Article Title: Huo Xue Tong Luo capsule epigenetically activates β-catenin, promotes Prx1-lineage cells proliferation and endochondral bone formation during fracture healing Article Snippet: The animals were housed in the SPF-grade experimental animal center of the First Affiliated Hospital of Guangzhou University of Chinese Medicine, with the license number SCXK (Guangdong) 2023-0092. .. Small animal X-Ray imager (Faxitron X-Ray, Tucson, AZ, USA); Small animal micro-CT (viva CT 40); Biosafety cabinet, cell culture incubator, ultra-low- temperature freezer, Varioskan LUX multi-function microplate reader (Thermo); Quantitative fluorescence PCR instrument (BioRAD); Polyvinylidene fluoride (PVDF) membrane (Millipore); Paraffin microtome, cryostat microtome, image information acquisition system (Leica); Fetal bovine serum (Gibco Cat NO. 10091-48); penicillin/streptomycin (Cytiva, HyClone Cat NO. SV30010), 0.25% trypsin (Cytiva, HyClone Cat NO. SV30031.01); Huo Xue Tong Luo capsule (Approval No. Z20071224 of Guangdong Provincial Medicine Preparation, the First Affiliated Hospital of GZUCM); β-Glycerophosphate disodium salt (Solarbio, Cat NO. G8100); Dexamethasone (Sigma, Cat NO. D4902-100MG); Ascorbic acid (Sangon Biotec, Cat NO. A610021); Alizarin red (Solarbio, Cat NO. G1450); ALP (Sigma, Cat NO. 86 C-1KT); Cell Counting Kit-8 (CCK-8) for cell proliferation detection (Solarbio, Cat NO. CA1210); Polymerase Chain Reaction:Article Title: Cyasterone Improves Mitochondrial Function and Protects against Knee Osteoarthritis by Activating PPARγ. Article Snippet: Beijing University of Chinese Medicine Shenzhen Hospital (Longgang), Shenzhen, 518172, P.R.. China; Key Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510080, P.R.. China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science and Innovation, CAS Ltd, Fifth Affiliated Hospital, Guangzhou Medical University, P.R. Article Title: Huo Xue Tong Luo capsule epigenetically activates β-catenin, promotes Prx1-lineage cells proliferation and endochondral bone formation during fracture healing Article Snippet: The animals were housed in the SPF-grade experimental animal center of the First Affiliated Hospital of Guangzhou University of Chinese Medicine, with the license number SCXK (Guangdong) 2023-0092. .. Small animal X-Ray imager (Faxitron X-Ray, Tucson, AZ, USA); Small animal micro-CT (viva CT 40); Biosafety cabinet, cell culture incubator, ultra-low- temperature freezer, Varioskan LUX multi-function microplate reader (Thermo); Quantitative fluorescence PCR instrument (BioRAD); Polyvinylidene fluoride (PVDF) membrane (Millipore); Paraffin microtome, cryostat microtome, image information acquisition system (Leica); Fetal bovine serum (Gibco Cat NO. 10091-48); penicillin/streptomycin (Cytiva, HyClone Cat NO. SV30010), 0.25% trypsin (Cytiva, HyClone Cat NO. SV30031.01); Huo Xue Tong Luo capsule (Approval No. Z20071224 of Guangdong Provincial Medicine Preparation, the First Affiliated Hospital of GZUCM); β-Glycerophosphate disodium salt (Solarbio, Cat NO. G8100); Dexamethasone (Sigma, Cat NO. D4902-100MG); Ascorbic acid (Sangon Biotec, Cat NO. A610021); Alizarin red (Solarbio, Cat NO. G1450); ALP (Sigma, Cat NO. 86 C-1KT); Cell Counting Kit-8 (CCK-8) for cell proliferation detection (Solarbio, Cat NO. CA1210); Membrane:Article Title: Cyasterone Improves Mitochondrial Function and Protects against Knee Osteoarthritis by Activating PPARγ. Article Snippet: Beijing University of Chinese Medicine Shenzhen Hospital (Longgang), Shenzhen, 518172, P.R.. China; Key Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510080, P.R.. China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science and Innovation, CAS Ltd, Fifth Affiliated Hospital, Guangzhou Medical University, P.R. Article Title: Huo Xue Tong Luo capsule epigenetically activates β-catenin, promotes Prx1-lineage cells proliferation and endochondral bone formation during fracture healing Article Snippet: The animals were housed in the SPF-grade experimental animal center of the First Affiliated Hospital of Guangzhou University of Chinese Medicine, with the license number SCXK (Guangdong) 2023-0092. .. Small animal X-Ray imager (Faxitron X-Ray, Tucson, AZ, USA); Small animal micro-CT (viva CT 40); Biosafety cabinet, cell culture incubator, ultra-low- temperature freezer, Varioskan LUX multi-function microplate reader (Thermo); Quantitative fluorescence PCR instrument (BioRAD); Polyvinylidene fluoride (PVDF) membrane (Millipore); Paraffin microtome, cryostat microtome, image information acquisition system (Leica); Fetal bovine serum (Gibco Cat NO. 10091-48); penicillin/streptomycin (Cytiva, HyClone Cat NO. SV30010), 0.25% trypsin (Cytiva, HyClone Cat NO. SV30031.01); Huo Xue Tong Luo capsule (Approval No. Z20071224 of Guangdong Provincial Medicine Preparation, the First Affiliated Hospital of GZUCM); β-Glycerophosphate disodium salt (Solarbio, Cat NO. G8100); Dexamethasone (Sigma, Cat NO. D4902-100MG); Ascorbic acid (Sangon Biotec, Cat NO. A610021); Alizarin red (Solarbio, Cat NO. G1450); ALP (Sigma, Cat NO. 86 C-1KT); Cell Counting Kit-8 (CCK-8) for cell proliferation detection (Solarbio, Cat NO. CA1210); ATP Assay:Article Title: Cyasterone Improves Mitochondrial Function and Protects against Knee Osteoarthritis by Activating PPARγ. Article Snippet: Beijing University of Chinese Medicine Shenzhen Hospital (Longgang), Shenzhen, 518172, P.R.. China; Key Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510080, P.R.. China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science and Innovation, CAS Ltd, Fifth Affiliated Hospital, Guangzhou Medical University, P.R. Reverse Transcription:Article Title: Cyasterone Improves Mitochondrial Function and Protects against Knee Osteoarthritis by Activating PPARγ. Article Snippet: Beijing University of Chinese Medicine Shenzhen Hospital (Longgang), Shenzhen, 518172, P.R.. China; Key Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510080, P.R.. China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science and Innovation, CAS Ltd, Fifth Affiliated Hospital, Guangzhou Medical University, P.R. Article Title: Huo Xue Tong Luo capsule epigenetically activates β-catenin, promotes Prx1-lineage cells proliferation and endochondral bone formation during fracture healing Article Snippet: The animals were housed in the SPF-grade experimental animal center of the First Affiliated Hospital of Guangzhou University of Chinese Medicine, with the license number SCXK (Guangdong) 2023-0092. .. Small animal X-Ray imager (Faxitron X-Ray, Tucson, AZ, USA); Small animal micro-CT (viva CT 40); Biosafety cabinet, cell culture incubator, ultra-low- temperature freezer, Varioskan LUX multi-function microplate reader (Thermo); Quantitative fluorescence PCR instrument (BioRAD); Polyvinylidene fluoride (PVDF) membrane (Millipore); Paraffin microtome, cryostat microtome, image information acquisition system (Leica); Fetal bovine serum (Gibco Cat NO. 10091-48); penicillin/streptomycin (Cytiva, HyClone Cat NO. SV30010), 0.25% trypsin (Cytiva, HyClone Cat NO. SV30031.01); Huo Xue Tong Luo capsule (Approval No. Z20071224 of Guangdong Provincial Medicine Preparation, the First Affiliated Hospital of GZUCM); β-Glycerophosphate disodium salt (Solarbio, Cat NO. G8100); Dexamethasone (Sigma, Cat NO. D4902-100MG); Ascorbic acid (Sangon Biotec, Cat NO. A610021); Alizarin red (Solarbio, Cat NO. G1450); ALP (Sigma, Cat NO. 86 C-1KT); Cell Counting Kit-8 (CCK-8) for cell proliferation detection (Solarbio, Cat NO. CA1210); Article Title: Cnidium monnieri Polysaccharides Exhibit Inhibitory Effect on Airborne Transmission of Influenza A Virus. Article Snippet: Total RNA was extracted from mouse lung tissue using the RNeasy Plus Universal Kit (73404, Qiagen, Germany). .. Reverse transcription was performed using the Article Title: "Alpha ketoglutarate mediated regulation of maturation and oxidative balance" in OPU derived Bos indicus oocytes. Article Snippet: Alpha-Ketoglutarate (AKG), a pivotal metabolite in the tricarboxylic acid cycle, possesses notable antioxidant properties that reduce oxidative stress.. While its benefits in in-vitro maturation (IVM) of porcine, murine, and ovine oocytes are documented, its role in bovine oocytes—especially that derived via ovum pick-up (OPU)— remains unexamined.. This is the first study to use OPU-derived oocytes from Sahiwal cattle (Bos indicus) to test 15 μM, 30 μM, and 45 μM AKG concentrations during IVM. Article Title: Cnidium monnieri Polysaccharides Exhibit Inhibitory Effect on Airborne Transmission of Influenza A Virus Article Snippet: Total RNA was extracted from mouse lung tissue using the RNeasy Plus Universal Kit (73404, Qiagen, Germany). .. Reverse transcription was performed using the Multiple Displacement Amplification:Article Title: Cyasterone Improves Mitochondrial Function and Protects against Knee Osteoarthritis by Activating PPARγ. Article Snippet: Beijing University of Chinese Medicine Shenzhen Hospital (Longgang), Shenzhen, 518172, P.R.. China; Key Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510080, P.R.. China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science and Innovation, CAS Ltd, Fifth Affiliated Hospital, Guangzhou Medical University, P.R. Quantitative RT-PCR:Article Title: Onnamides A and B Suppress Hepatitis B Virus Transcription by Inhibiting Viral Promoter Activity. Article Snippet: .. Subsequently, quantitative RT-qPCR was performed using Article Title: Onnamides A and B Suppress Hepatitis B Virus Transcription by Inhibiting Viral Promoter Activity Article Snippet: .. Subsequently, quantitative RT-qPCR was performed using Reverse Transcription Polymerase Chain Reaction:Article Title: Onnamides A and B Suppress Hepatitis B Virus Transcription by Inhibiting Viral Promoter Activity. Article Snippet: .. Subsequently, quantitative RT-qPCR was performed using Article Title: Identification and knockdown effect of disulfide isomerase in the Haemaphysalis longicornis (Acari: Ixodidae) Article Snippet: .. Real-time PCR was performed to determine gene expression after knockdown, using a Article Title: Onnamides A and B Suppress Hepatitis B Virus Transcription by Inhibiting Viral Promoter Activity Article Snippet: .. Subsequently, quantitative RT-qPCR was performed using Real-time Polymerase Chain Reaction:Article Title: Identification and knockdown effect of disulfide isomerase in the Haemaphysalis longicornis (Acari: Ixodidae) Article Snippet: .. Real-time PCR was performed to determine gene expression after knockdown, using a Gene Expression:Article Title: Identification and knockdown effect of disulfide isomerase in the Haemaphysalis longicornis (Acari: Ixodidae) Article Snippet: .. Real-time PCR was performed to determine gene expression after knockdown, using a Knockdown:Article Title: Identification and knockdown effect of disulfide isomerase in the Haemaphysalis longicornis (Acari: Ixodidae) Article Snippet: .. Real-time PCR was performed to determine gene expression after knockdown, using a Micro-CT:Article Title: Huo Xue Tong Luo capsule epigenetically activates β-catenin, promotes Prx1-lineage cells proliferation and endochondral bone formation during fracture healing Article Snippet: The animals were housed in the SPF-grade experimental animal center of the First Affiliated Hospital of Guangzhou University of Chinese Medicine, with the license number SCXK (Guangdong) 2023-0092. .. Small animal X-Ray imager (Faxitron X-Ray, Tucson, AZ, USA); Small animal micro-CT (viva CT 40); Biosafety cabinet, cell culture incubator, ultra-low- temperature freezer, Varioskan LUX multi-function microplate reader (Thermo); Quantitative fluorescence PCR instrument (BioRAD); Polyvinylidene fluoride (PVDF) membrane (Millipore); Paraffin microtome, cryostat microtome, image information acquisition system (Leica); Fetal bovine serum (Gibco Cat NO. 10091-48); penicillin/streptomycin (Cytiva, HyClone Cat NO. SV30010), 0.25% trypsin (Cytiva, HyClone Cat NO. SV30031.01); Huo Xue Tong Luo capsule (Approval No. Z20071224 of Guangdong Provincial Medicine Preparation, the First Affiliated Hospital of GZUCM); β-Glycerophosphate disodium salt (Solarbio, Cat NO. G8100); Dexamethasone (Sigma, Cat NO. D4902-100MG); Ascorbic acid (Sangon Biotec, Cat NO. A610021); Alizarin red (Solarbio, Cat NO. G1450); ALP (Sigma, Cat NO. 86 C-1KT); Cell Counting Kit-8 (CCK-8) for cell proliferation detection (Solarbio, Cat NO. CA1210); Hood:Article Title: Huo Xue Tong Luo capsule epigenetically activates β-catenin, promotes Prx1-lineage cells proliferation and endochondral bone formation during fracture healing Article Snippet: The animals were housed in the SPF-grade experimental animal center of the First Affiliated Hospital of Guangzhou University of Chinese Medicine, with the license number SCXK (Guangdong) 2023-0092. .. Small animal X-Ray imager (Faxitron X-Ray, Tucson, AZ, USA); Small animal micro-CT (viva CT 40); Biosafety cabinet, cell culture incubator, ultra-low- temperature freezer, Varioskan LUX multi-function microplate reader (Thermo); Quantitative fluorescence PCR instrument (BioRAD); Polyvinylidene fluoride (PVDF) membrane (Millipore); Paraffin microtome, cryostat microtome, image information acquisition system (Leica); Fetal bovine serum (Gibco Cat NO. 10091-48); penicillin/streptomycin (Cytiva, HyClone Cat NO. SV30010), 0.25% trypsin (Cytiva, HyClone Cat NO. SV30031.01); Huo Xue Tong Luo capsule (Approval No. Z20071224 of Guangdong Provincial Medicine Preparation, the First Affiliated Hospital of GZUCM); β-Glycerophosphate disodium salt (Solarbio, Cat NO. G8100); Dexamethasone (Sigma, Cat NO. D4902-100MG); Ascorbic acid (Sangon Biotec, Cat NO. A610021); Alizarin red (Solarbio, Cat NO. G1450); ALP (Sigma, Cat NO. 86 C-1KT); Cell Counting Kit-8 (CCK-8) for cell proliferation detection (Solarbio, Cat NO. CA1210); Cell Culture:Article Title: Huo Xue Tong Luo capsule epigenetically activates β-catenin, promotes Prx1-lineage cells proliferation and endochondral bone formation during fracture healing Article Snippet: The animals were housed in the SPF-grade experimental animal center of the First Affiliated Hospital of Guangzhou University of Chinese Medicine, with the license number SCXK (Guangdong) 2023-0092. .. Small animal X-Ray imager (Faxitron X-Ray, Tucson, AZ, USA); Small animal micro-CT (viva CT 40); Biosafety cabinet, cell culture incubator, ultra-low- temperature freezer, Varioskan LUX multi-function microplate reader (Thermo); Quantitative fluorescence PCR instrument (BioRAD); Polyvinylidene fluoride (PVDF) membrane (Millipore); Paraffin microtome, cryostat microtome, image information acquisition system (Leica); Fetal bovine serum (Gibco Cat NO. 10091-48); penicillin/streptomycin (Cytiva, HyClone Cat NO. SV30010), 0.25% trypsin (Cytiva, HyClone Cat NO. SV30031.01); Huo Xue Tong Luo capsule (Approval No. Z20071224 of Guangdong Provincial Medicine Preparation, the First Affiliated Hospital of GZUCM); β-Glycerophosphate disodium salt (Solarbio, Cat NO. G8100); Dexamethasone (Sigma, Cat NO. D4902-100MG); Ascorbic acid (Sangon Biotec, Cat NO. A610021); Alizarin red (Solarbio, Cat NO. G1450); ALP (Sigma, Cat NO. 86 C-1KT); Cell Counting Kit-8 (CCK-8) for cell proliferation detection (Solarbio, Cat NO. CA1210); cDNA Synthesis:Article Title: "Alpha ketoglutarate mediated regulation of maturation and oxidative balance" in OPU derived Bos indicus oocytes. Article Snippet: Alpha-Ketoglutarate (AKG), a pivotal metabolite in the tricarboxylic acid cycle, possesses notable antioxidant properties that reduce oxidative stress.. While its benefits in in-vitro maturation (IVM) of porcine, murine, and ovine oocytes are documented, its role in bovine oocytes—especially that derived via ovum pick-up (OPU)— remains unexamined.. This is the first study to use OPU-derived oocytes from Sahiwal cattle (Bos indicus) to test 15 μM, 30 μM, and 45 μM AKG concentrations during IVM. |